Beloved Readers,

I am still trying to figure out how to make a living, despite all of my accomplishments!  Here is my CV.  Thank you for your consideration.

Lots of Love, Marie

Dr. Marie D. Kube, Ph.D.

2038 Ford Parkway, #369, St. Paul, MN, 55116

www.art-and-smart.com/contact

Education

Ph.D., Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN, 1997, GPA 3.98/4.00

B.A., Chemistry (with minor in Mathematics), with distinction, University of Colorado at Denver, CO, 1990, GPA 3.87/4.00

Professional Experience

Author & Model                                                                                                            2011-Present

– Author, model, and photographer for Sexiest at 50: PTSD PhD Marie, edited by Dr. Michael G. Klug, Ph.D., a proven guide for healthy living to age 50, available here and here

– Author of Instructions for Helping to Improve the Human Condition, a journey into the spiritual realm from a non-religious, scientifically trained perspective, book available here and here

– Publisher of Art and Smart, edited by Dr. Michael G. Klug, Ph.D., a magazine dedicated to the Art of Living Smart, available here and here

– Creator of websites dedicated to the confluence of science and spirituality:  www.art-and-smart.com, with at least 20 blog posts, and www.myspiritualassociation.com, with mature content and at least 25 blog posts

Volunteer                                                                                                                             2009-2016

Minneapolis Community & Technical College (MCTC), Minneapolis, MN

– Assisted Dr. Michael G. Klug in developing and updating the curriculum and assessments, in grading, and in the preparations for his Molecular Biology BIOL 2500 course and laboratory that he taught Spring 2010, 2011, 2012, 2013, 2014, 2015, 2016

– Assisted Dr. Michael G. Klug with anything and everything I could do to promote success of students, colleagues, and MCTC

 Consultant                                                                                                                          2010-2011

University of Minnesota, Minneapolis, MN

– Consultant for development and commercialization of new technology

Director of Development                                                                                                2007-2010

VitalMedix, Inc., Hudson, WI

– Developed a parenteral drug formulation for severe blood loss

– Planned and managed outsourcing of active pharmaceutical ingredient manufacturing, analytical assays, and pre-clinical safety & efficacy studies

– Coordinated with team members, external contractors and consultants for Pre-Investigational New Drug (Pre-IND) meeting with the FDA, including writing a well-received submission document with pre-clinical and clinical study plans

– Prepared and delivered technical presentations for current and potential investors and other stakeholders

– Wrote grant applications

Patent Agent (Reg. No. 59,744)                                                                                       2005-2007

Fish & Richardson P.C., Minneapolis, MN

– Prepared and prosecuted numerous patent applications in the field of biotechnology

– Participated in patentability, due diligence, and freedom to operate analyses

Research Associate                                                                                                           2003-2005

Mayo Clinic, Rochester, MN

– Performed research for the prostate cancer biomarker discovery project of the Minnesota Partnership for Biotechnology and Medical Genomics

– Developed, optimized, and validated molecular biological methods

– Coordinated a multi-disciplinary team (including pathologists, bioinformaticians, molecular biologists, and protein biochemists) to achieve project goals within tight deadlines

– Generated, analyzed, presented, and published results

Contributing Editor                                                                                                          2002-2003

Reed Business Information, Morris Plains, NJ

– Published 16 articles about cutting edge technologies in Drug Discovery & Development and Genomics & Proteomics trade journals

– Interviewed high-level executives in small, medium, and large biotechnology and pharmaceutical companies

 Laboratory Head and Project Leader                                                                        1999-2002

Hoffmann-La Roche, Basel, Switzerland

– Project leader of a drug discovery and development program, involving collaborations across departments and sites (Palo Alto, CA and Basel, Switzerland)

– Designed pre-clinical testing strategies

– Trained and supervised technicians

– Developed and validated in vitro assays

– Screened and characterized compounds

– Presented results to peers, management, and external scientific experts, advisors, and consultants

– Conducted research in obesity and type 2 diabetes as a postdoctoral researcher prior to being promoted

– Applied genomics (real-time, quantitative PCR and microarray technology) to study hypothalamic control of food intake in rodent models of food deprivation and overeating

– Inventor on U.S. Patent No. 6,900,227; U.S. Patent Application Publication No. 20050143373; and International Patent Application Publication No. WO2004013120

Instructor                                                                                                                            1994-1996

Indiana University School of Medicine, Indianapolis, IN

– Facilitator for the Problem Based Learning Section of Medical Microbiology J601 for medical students (8/1996 to 12/1996)

– Instructor for Nursing Microbiology Lab J210 (1/1996 to 5/1996 and 8/1994 to 12/1994)

Associate Pharmaceutical Chemist                                                                             1990-1993

Lilly Research Laboratories, Eli Lilly and Co., Indianapolis, IN

– Developed the formulation for Humalog®

– Supported manufacture of clinical trial batches

– Provided supporting documentation for FDA approval

– Collaborated with statisticians to apply multivariate statistical methods to design experiments and analyze data

– Performed research on protein aggregation and protein crystal agglomeration in relation to physical stability of protein products

Honors and Awards

– Esther L. Kinsley Ph.D. Dissertation Award, Indiana University.  This is the highest honor for research that Indiana University bestows upon its graduate students.  Indiana University awarded 336 Ph.D.s in 1997 across all disciplines, and only one degree recipient was given the Kinsley Award.

– Travel Fellowship, Committee on Institutional Cooperation’s Women in Science and Engineering Initiative, 1997

– Sigma Xi Graduate Student Research Competition, Indiana University School of Medicine, Honorable Mention, 1996

– Travel Fellowship, Indiana University Purdue University at Indianapolis Fellowship Committee, 1995

– University Graduate Fellowship, Indiana University, 1994

– National Science Foundation Graduate Fellowship, Honorable Mention, 1994

– Outstanding Chemistry Student, The American Institute of Chemists Foundation, 1991

– Outstanding Undergraduate Student in the College of Liberal Arts and Sciences, University of Colorado at Denver, Finalist, 1991

– H.A. Arnold Scholarship, 1989-1990

– Colorado Dean’s List Scholarship, 1986-1987

– Regent’s Scholarship, 1985-1986

Publications (in chronological order)

A. Peer-Reviewed Journal Articles

Kube, D.M., and A. Srivastava. (1997) Quantitative DNA Slot Blot Analysis: Inhibition of DNA Binding to Membranes by Magnesium Ions. Nucleic Acids Res., 25:3375.

Kube, D.M., S. Ponnazhagan, and A. Srivastava. (1997) Encapsidation of Adeno-Associated Virus Type 2 Rep Proteins in Wild-Type and Recombinant Progeny Virions: Rep-Mediated Growth Inhibition of Primary Human Cells. J. Virol., 71:7361.

Qing, K., X.-S. Wang, D.M. Kube, S. Ponnazhagan, A. Bajpai, and A. Srivastava. (1997) Role of Tyrosine Phosphorylation of a Cellular Protein in Adeno-Associated Virus Type 2-Mediated Transgene Expression. Proc. Natl. Acad. Sci. USA, 94:10879.

Ponnazhagan, S., P. Mukherjee, X.-S. Wang, K. Qing, D.M. Kube, C. Mah, C. Kurpad, M.C. Yoder, E.F. Srour, and A. Srivastava. (1997) Adeno-Associated Virus Type 2-Mediated Transduction in Primary Human Bone Marrow-Derived CD34⁺ Hematopoietic Progenitor Cells: Donor Variation and Correlation of Transgene Expression with Cellular Differentiation. J. Virol., 71:8262.

Qing, K., B. Khuntirat, C. Mah, D.M. Kube, X.-S. Wang, S. Ponnazhagan, S. Zhou, V.J. Dwarki, M.C. Yoder, and A. Srivastava. (1998) Adeno-Associated Virus Type 2-Mediated Gene Transfer: Correlation of Tyrosine Phosphorylation of the Cellular Single-Stranded D Sequence-Binding Protein with Transgene Expression in Human Cells In Vitro and Murine Tissues In Vivo. J. Virol., 72:1593.

Wang, X.-S., B. Khuntirat, K. Qing, S. Ponnazhagan, D.M. Kube, S. Zhou, V.J. Dwarki, and A. Srivastava. (1998) Characterization of Wild-Type Adeno-Associated Virus Type 2-Like Particles Generated During Recombinant Viral Vector Production and Strategies for Their Elimination. J. Virol., 72:5472.

Mah, C., K. Qing, B. Khuntirat, S. Ponnazhagan, X.-S. Wang, D.M. Kube, M.C. Yoder, and A. Srivastava. (1998) Adeno-Associated Virus Type 2-Mediated Gene Transfer: Role of Epidermal Growth Factor Receptor Protein Tyrosine Kinase in Transgene Expression. J. Virol., 72:9835.

Rogers-Evans, M., A.I. Alanine, K.H. Bleicher, D. Kube, and G. Schneider. (2004) Identification of novel cannabinoid receptor ligands via evolutionary de novo design and rapid parallel synthesis. QSAR & Combinatorial Science, 23(6):426.

Kosari, F., A.S. Parker, D.M. Kube, C.M. Lohse, B.C. Leibovich, M.L. Blute, J.C. Cheville, and G. Vasmatzis. (2005) Clear Cell Renal Cell Carcinoma: Gene Expression Analyses Identify a Potential Signature for Tumor Aggressiveness. Clin. Cancer Res., 11:5128.

Kube, D.M., C.D. Savci-Heijink, A.-F. Lamblin, F. Kosari, G. Vasmatzis, J.C. Cheville, D.P. Connelly, and G.G. Klee. (2007) Optimization of Laser Capture Microdissection and RNA Amplification for Gene Expression Profiling of Prostate Cancer. BMC Molecular Biology, 8:25.

Vasmatzis, G., E. Klee, D.M. Kube, T. Therneau, and F. Kosari. (2007) Quantitating Tissue Specificity of Human Genes to Facilitate Biomarker Discovery.  Bioinformatics, 23:1348.

Kosari, F., J.M.A. Munz, C.D. Savci-Heijink, C. Spiro, E.W. Klee, D.M. Kube, L. Tillmans, J. Slezak, R.J. Karnes, J.C. Cheville, and G. Vasmatzis. (2008) Identification of Prognostic Biomarkers for Prostate Cancer. Clin. Cancer Res., 14:1734.

Alig, L., J. Alsenz, M. Andjelkovic, S. Bendels, A. Bénardeau, K. Bleicher, A. Bourson, P. David-Pierson, W. Guba, S. Hildbrand, D. Kube, T. Lübbers, A. Mayweg, R. Narquizian, W. Neidhart, M. Nettekoven, J-.M. Plancher, C. Rocha, M. Rogers-Evans, S. Röver, G. Schneider, S. Taylor, and P. Waldmeier. (2008) Benzodioxoles:  Novel Cannabinoid-1 Receptor Inverse Agonists for the Treatment of Obesity.  J. Med. Chem., 51(7):2115.

 B. Technical Reports

Kube, D.M. Chemical Genomics Energizes Discovery. Drug Discovery & Development, March 2002, Vol.5, No.3, p45.

Kube, D.M. Mining for Molecular Interactions. Genomics & Proteomics, April 2002.

Kube, D.M. Automatic DNA Purification and Sequencing Evolve. Drug Discovery & Development, July/August 2002, Vol.5, No.7, p38.

Kube, D.M. Genomics and Proteomics Continue to Pave the Way to the Clinic. Genomics & Proteomics, July/August 2002.

Kube, D.M. Multitalented Proteins Play a Key Role in Therapeutics. Genomics & Proteomics, September 2002.

Kube, D.M. A Model Approach to Streamline Drug Discovery. Drug Discovery & Development, October 2002, Vol.5, No.9, p59.

Kube, D.M. OLA, MDI, PEG: the latest words in drug delivery. Drug Discovery & Development, November 2002, Vol.5, No.10, p81.

Kube, D.M. Good Manufacturing Practice is Getting Better. Drug Discovery & Development, December 2002, Vol.5, No.11, p63.

Kube, D.M. Picking Potential Drugs with In Vitro ADMET Models. Drug Discovery & Development, January 2003, Vol.6, No.1, p50.

Kube, D.M. Metabonomics Treats Attrition in Early Drug Discovery. Drug Discovery & Development, March 2003, Vol.6, No.3, p45.

Kube, D.M. Profiles of Blockbuster Drugs and Effective Drug Targets. Drug Discovery & Development, March 2003, Vol.6, No.3, p73.

Kube, D.M. A Multidisciplinary Approach to Target Validation. Drug Discovery & Development, April 2003, Vol.6, No.4, p37.

Kube, D.M. Innovative Assay Platforms Drive Proteomics Research. Drug Discovery & Development, April 2003, Vol.6, No.4, p45.

Kube, D.M. Mass Spectrometry: Drug Discovery’s Essential Tool. Drug Discovery & Development, May 2003, Vol.6, No.5, p71.

Kube, D.M. Power of Automated Protein Fractionation. Genomics & Proteomics, May 2003, Vol.3, No.4, p36.

Kube, D.M. Rational Drug Design Enables Informed Decision-Making. Drug Discovery & Development, June 2003.

C. Presentations and Published Abstracts

Kim, Y., and D.M. Kube. (1991) Doppler Electrophoretic Light Scattering Analyzer for Determination of the Zeta Potential of Human Insulin Particles in Suspension. Lilly Research Labs Symposium, Indianapolis, IN.

Kube, D.M., S. Ponnazhagan, and A. Srivastava. (1995) AAV-Mediated Cytopathic Effect on Primary Human Bone Marrow Stromal Cells. VIth Parvovirus Workshop, Montpellier, France.

Srivastava, A., X.-S. Wang, S. Ponnazhagan, S.Z. Zhou, D.M. Kube, and M. Yoder. (1995) Parvovirus-Based Vectors for Human Gene Therapy. VIth Parvovirus Workshop, Montpellier, France.

Kube, D.M., S. Ponnazhagan, and A. Srivastava. (1995) Encapsidation of the Adeno-Associated Virus Type 2 Rep Proteins in Progeny Virions: Rep-Mediated Growth Inhibition of Primary Human Bone Marrow Stromal Cells. Blood, 86(10):3990.

Kube, D.M., S. Ponnazhagan, and A. Srivastava. (1997) Encapsidation of AAV Rep Proteins in Progeny Virions: Wild-Type and Recombinant AAV-Mediated Growth Inhibition of Primary Human Cells. International Parvovirus Workshop, Heidelberg, Germany.

Qing, K.Y., B. Khuntirat, C. Mah, D.M. Kube, X.-S. Wang, S. Ponnazhagan, S.Z. Zhou, V. Dwarki, M.C. Yoder, and A. Srivastava. (1997) Adeno-Associated Virus-Mediated Gene Transfer: Correlation of Tyrosine Phosphorylation with Transgene Expression in Human Cells In Vitro and Murine Tissues In Vivo. Blood, 90(10):525.

Qing, K., X.-S. Wang, D.M. Kube, S. Ponnazhagan, A. Bajpai, and A. Srivastava. (1997) Role of Tyrosine Phosphorylation of a Cellular Protein in Adeno-Associated Virus Type 2-Mediated Transgene Expression. International Parvovirus Workshop, Heidelberg, Germany. (Blood, 90(10):526).

Ponnazhagan, S., P. Mukherjee, X.-S. Wang, K. Qing, D.M. Kube, C. Mah, C. Kurpad, M.C. Yoder, E.F. Srour, and A. Srivastava. (1997) AAV-Mediated Transduction of Primary Human Bone Marrow-Derived CD34+ Cells: Donor Variation and Correlation of Transgene Expression with Cellular Differentiation. International Parvovirus Workshop, Heidelberg, Germany.

Srivastava, A., S. Ponnazhagan, X.-S. Wang, K. Qing, P. Mukherjee, B. Khuntirat, D.M. Kube, C. Mah, and M.C. Yoder. (1997) AAV and Parvovirus B19 Vectors for Human Gene Therapy. International Parvovirus Workshop, Heidelberg, Germany.

Mah, C., K.Y. Qing, B. Khuntirat, S. Ponnazhagan, X.-S. Wang, D.M. Kube, M.C. Yoder, and A. Srivastava. (1998) Adeno-associated Virus 2-Mediated Gene Transfer: Role of Epidermal Growth Factor Receptor Protein Tyrosine Kinase in Transgene Expression. Blood, 92(10):150A.

Weigel, K.A., D.M. Kube, and A. Srivastava. (1998) Human Endothelial Cell-Specific Transgene Expression Mediated by a Recombinant Adeno-Associated Virus 2-Parvovirus B19 Hybrid Vector. Blood, 92(10):382B.

Kube, D.M., C.D. Savci-Heijink, A.-F. Lamblin, G. Vasmatzis, J.C. Cheville, D.P. Connelly, and G.G. Klee. (2005) Optimization of Laser Capture Microdissection and RNA Amplification for Gene Expression Profiling of Prostate Cancer. American Association for Cancer Research, Anaheim, CA.

D. Patents

– Benzodioxole Derivatives, U.S. Patent No. 6,900,227, U.S. Patent No. 7,576,088, Hoffman-La Roche.

– Novel Benzodioxoles, International Patent Application Publication No. WO2004013120 (granted in Australia, European Patent Office, India, Republic of Korea and New Zealand), European Patent Specification No. EP 1 532 132, F. Hoffman-La Roche.