I Wanted to Be That Woman

April 19, 2018Leave a comment

When I was young and traveling with my family, I once saw a woman pull up in her car under the covered driveway along the front of a motel out west. She was all by herself. She was sexy and so was her car, yet neither was the most expensive one. Her boots kicked up the dust as she got out of the car, and then she put on her black felt cowboy hat over her dark shoulder-length hair. She was young and beautiful and confident in her blue jeans, like she knew exactly what she was doing. And she had grown hard and strong and tough, at least on the outside, wherever she had been planted. But she had no roots. And all the storms she had survived only made her stronger and could not take away her beauty or her charm or the sparkle of the excitement in her eyes that somehow coexisted with her wisdom, or at least street smarts. She seemed quite fit as she moved energetically, gracefully, and decisively. She was renting a room in a motel all by herself during a long drive. I felt so excited by her freedom as the gypsy in me awoke, and oh, I so wanted to be that woman…

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Michael Klug’s Curriculum Vitae

April 9, 2018April 9, 2018Leave a comment

Dear Readers,

Here is Michael Klug’s CV. As you know, he is also the editor and photographer for the books Sexiest at 50: PTSD PhD Marie (available at http://a.co/3UJckBL) and Art and Smart (available at http://a.co/49hSME1). You can also hear him using the Instructions for Helping to Improve the Human Condition with me as the guide at https://myspiritualconnection.org/instructions/recording-of-mary-michael-performing-service-using-the-instructions-for-helping-to-improve-the-human-condition/. The Instructions for Helping to Improve the Human Condition are freely available at http://www.art-and-smart.com and at https://myspiritualassociation.com/instructions-for-helping-to-improve-the-human-condition/. Printed books are also available at http://a.co/3LVufm5.

Lots of Love, Marie

Michael G. Klug, Ph.D.

Biology Instructor

Minneapolis Community & Technical College (MCTC)

1501 Hennepin Avenue

Minneapolis, MN 55403

https://www.linkedin.com/in/michael-klug-b7a7b58/

EDUCATION

Ph.D., Physiology, Indiana University School of Medicine, Indianapolis, IN, 1989-1996 (GPA 3.93 / 4.00)

B.S., Honors Biology program, with distinction, Phi Beta Kappa, Indiana University, Bloomington, Indiana, 1986-1989 (GPA 3.77 / 4.00)

Additional Graduate Level Coursework:

Successfully completed first two years of medical school (M.D.), Indiana University School of Medicine, Bloomington, IN, 1989-1991. (When I was starting back into my third year of medical school, I received a post-doc job offer in 1997 to continue my Ph.D. research and never looked back.)

Instructional Strategies for Community & Technical Colleges, Bemidji State University, Bemidji, Minnesota, 2010 (Grade: A).

Curriculum Planning and Design for Community & Technical Colleges, Bemidji State University, Bemidji, Minnesota, 2011 (Grade: A).

Assessment and Evaluation of Student Learning for Community & Technical Colleges, Bemidji State University, Bemidji, Minnesota, 2011 (Grade: A).

Philosophy of Community and Technical College Education, Minnesota State Colleges & Universities (MnSCU) Office of the Chancellor, 2012, (non-credit, required for MnSCU college faculty).

Additional Certification:

IDI (Intercultural Development Inventory), Qualified Administrator, August 2017 to present.

TEACHING EXPERIENCE

Instructor for BIOL 1100, Introduction to Biology class and laboratory sections, Minneapolis Community and Technical College (MCTC), Spring 2009.

Instructor for BIOL 1128, Human Biology laboratory, MCTC, Fall 2009, Spring 2017.

Instructor for BIOL 2200, Principles of Biology class sections, MCTC, Fall 2015.

Instructor for BIOL 2224, Human Anatomy class sections, MCTC, Fall 2009 to present.

Instructor for BIOL 2224, Human Anatomy laboratory sections, MCTC, Fall 2009 to present, (excluding parts of 2 years as Coordinator for several departments).

Instructor for BIOL 2224, Human Anatomy online laboratory sections, MCTC, Summer 2014, Fall 2014, Summer 2015.

Instructor for BIOL 2224, Human Anatomy online class sections, MCTC, Fall 2010 to present.

Instructor for BIOL 2225, Physiology online class section, MCTC, Summer 2013.

Instructor for BIOL 2225, Physiology laboratory sections, MCTC, Summer 2013, Fall 2017 to present.

Instructor for BIOL 2500, Molecular Biology class and laboratory, MCTC, Spring 2010, 2011, 2012, 2013, 2014, 2015, 2016.

Instructor for BIOT 1000, Introduction to Biosciences, MCTC, Fall 2010 to Spring 2015.

Teaching and Advising Experience Prior to Completion of Ph.D. degree:

Facilitator for the Problem Based Learning section of the Medical Physiology course, Indiana University School of Medicine, Spring 1994 and 1996.

Graduate student advisor to undergraduate Delta Chi fraternity members, 1989-1990 school year, Indiana University, Bloomington.

Associate Instructor for 2 sections of C121, undergraduate chemistry lab, Fall 1988, Indiana University, Bloomington.

COURSE DEVELOPMENT

Completely developed Molecular Biology course and laboratory (BIOL 2500). Includes a continuous, semester long laboratory project.

Completely developed online Anatomy course (BIOL 2224).

Completely developed online Anatomy laboratory (BIOL 2224).

Developed and continue to improve and optimize web and active group based enhanced learning opportunities for Anatomy courses and laboratory (BIOL 2224).

PROFESSIONAL EXPERIENCE

Summary:

Teaching while leveraging my diverse experiences in research, diagnostics, pharmaceuticals and biotechnology at the junction of life sciences, research, business development, product conception & development, and intellectual property.

Minneapolis Community and Technical College, Minneapolis, MN, USA 2009-present

Instructor for laboratories & classes in the Biology Department and Biotechnology Program.

Equity & Inclusion, Steering Committee, Fall 2016-Present
Equity & Inclusion, Curriculum & Pedagogy, Fall 2016-Fall 2017
Coordinator for Astronomy, Biology, Biotechnology, Chemistry, Community Health Worker, Geology, Herbal Studies and Physics, Fall 2014-Spring 2016
Faculty Racial Equity Committee
Safety Committee, 2009-2013

University of Minnesota, Minneapolis, MN, USA 2006-2008

Technology Strategy Manager and Technology Transfer Liaison, Academic Health Center

Identified new revenue opportunities and de-prioritized projects with limited potential.

New technology identification, evaluation, market analyses, pre-clinical and clinical development timeline estimation and management of intellectual property (patent) filing/prosecution.

– Worked with nearly 300 researchers to screen over 1000 invention ideas.

– Patent filing and prosecution including over 200 US and foreign patents.

– Created and developed relationships between the Office for Technology Commercialization and faculty, Center Directors, Department Heads, and College Deans.

– Identified potential commercialization steps for faculty research and introduced opportunities to commercial partners, University collaborators and funding sources.

– Educated Academic Health Center (Medical, Dental, Veterinary, and Public Health Colleges) faculty on intellectual property.

Mayo Clinic, Rochester, MN, USA 2003-2006

Licensing Liaison and Contract Manager (2004-2006)

Department of Laboratory Medicine and Pathology, Mayo Collaborative Services Inc., Mayo Medical Laboratories, Legal Contract Administration, Legal Department

Identified, categorized, prioritized and actualized hundreds of projects encompassing over a thousand near-term action items. Established the business case for the creation of 6+ new positions as well as in-licensing and collaborative opportunities.

– Results included negotiation of in-licensing and collaborative agreements and creation of a cohesive team that enabled new, and protected existing, revenue streams.

Research Fellow (2003-2004)

Department of Orthopedic Research, Mayo Graduate School of Medicine

Scientific research included proteomic analyses for discovery of arthritis biomarkers.

F. Hoffmann-La Roche Ltd., Basel, Switzerland 1999-2002

Laboratory Head, Project Leader, and Genomics and Genetics Coordinator (2000-2002)

Obesity and Type II Diabetes Drug Discovery

Planned, led, motivated, organized and contributed to teams composed of professionals with diverse expertise and personalities. Results included successful implementation of key projects/collaborations and re-design or de-prioritization of unfavorable opportunities. Contributed substantively to external collaborations.

– Led and coordinated a large network of colleagues to patent potential drugs that decreased food intake and could be used to treat obesity. Overall project plans included marketing, business development, clinical trials, regulatory and other aspects of pharmaceutical discovery, development and product life cycle.

– Attended Leadership and Teamwork Course, International Institute for Management Development (IMD International), Lausanne, Switzerland.

Laboratory Head and Project Leader (1999-2000)

New Target Discovery, Congestive Heart Failure Disease Area

Planned and implemented development of new research projects and facilities.

– Developed numerous proposals for new heart failure drug projects focused on competitive improvements over existing or future therapies.

– Co-designed and supervised construction of molecular and cellular biology laboratories.

– Contributed to planning and personnel recruiting for congestive heart failure pre-clinical model laboratories.

– Contributed to plans submitted to regulatory agencies for identification and management of potential cardiovascular risks in marketed drugs.

Eli Lilly and Company, Indianapolis, IN 1997-1999

Postdoctoral Fellow

Congestive Heart Failure Drug Discovery Team, Cardiovascular Division, Lilly Research Laboratories

Established and expanded new research area including supervision of design and construction of new laboratory facilities and purchase of new equipment. Provided review of plans and data analysis for collaborating scientists in a team based environment that resulted in more efficient integration of projects.

– Designed and implemented in vitro heart cell models that optimized the drug target discovery process.

– Transferred know-how and contributed to conceptualization, planning, evaluation and ongoing interactions within team and with other Lilly Divisions, external academic and biotechnology partners.

– Recruited, trained and supervised scientists.

Indiana University School of Medicine, Bloomington; Indianapolis, IN 1989-1996

Medical and Graduate Student (M.D./Ph.D.)

Completed first 2 years of Medical School and passed first National Board Exam.

Ph.D. Coursework and Dissertation Research (1991-1996)

Breakthrough scientific research establishing that heart cells from mouse embryonic stem cells could form grafts in adult hearts.

PUBLICATIONS and PRESENTATIONS

Jensen, M., Matteis, A., Loyle, A. Contributors: Cramer, S., Felice, J., Gerrits, R., Henniger, A., Jackson, J., Klug, M., Millis, L., Parsons, A., Ross, T. Fifteen POGIL Activities for Introductory Anatomy and Physiology Courses. 2014. Wiley.

RESEARCH ARTICLES

Bergen III, H. R. Klug, M.G., Bolander, M.E., Muddiman, D. C. Informed use of protease inhibitors in biomarker discovery. Rapid Commun. Mass Spectrom. 2004. 18:1001-2.

Wang, J., Zhen L., Klug, M.G., Wood D., Wu X., Mizrahi J. Involvement of caspase 3- and 8-like proteases in ceramide-induced apoptosis of cardiomyocytes. J. Card. Fail. 2000. 6:243-9.

Sutton, J., Costa, R., Klug, M., Field, L., Xu, D., Largaispada, D.A., Fletcher C.F., Jenkins, N.A., Copeland, N.G., Klemsz, M., and Hromas, R. Genesis, a winged helix transcriptional repressor with expression restricted to embryonic stem cells. J. Biol. Chem. 1996. 271:23126-23133. Cited over 75 times*

Klug, M.G., Soonpaa, M.H., Koh, G.Y., and Field, L.J. Genetically selected cardiomyocytes from differentiating embryonic stem cells form stable intracardiac grafts. J. Clin. Invest. 1996. 98:216-214. Cited over 400 times*

Klug, M.G., Soonpaa, M.H., and Field, L.J. DNA synthesis and multinucleation in embryonic stem cell-derived cardiomyocytes. Am. J. Physiol. 1995. 269 (Heart Circ. Physiol. 38):H1913-H1921. Cited over 25 times*

Koh, G.Y., Soonpaa, M.H., Klug, M.G., Pride, H.P., Cooper, B.J., Zipes, D.P., and Field, L.J. Stable fetal cardiomyocyte grafts in the hearts of dystrophic mice and dogs. J. Clin. Invest. 1995. 96:2034-2042. Cited over 100 times*

Koh, G.Y., Kim, S.J., Klug, M.G., Park, K., Soonpaa, M.H., and Field, L.J. Targeted expression of transforming growth factor-b1 in intracardiac grafts promotes vascular endothelial cell DNA synthesis. J. Clin. Invest. 1995. 95:114-121. Cited over 50 times*

Soonpaa, M.H., Koh, G.Y., Klug, M.G., and Field, L.J. Formation of nascent intercalated disks between grafted fetal cardiomyocytes and host myocardium. Science. 1994. 264:98-101. Cited over 200 times*

Koh, G.Y., Klug, M.G., Soonpaa, M.H., and Field, L.J. Differentiation and long-term survival of C2C12 myoblast grafts in the heart. J. Clin. Invest. 1993. 92:1548-1554. Cited over 100 times*

Koh, G.Y., Soonpaa, M.H., Klug, M.G., and Field, L.J. Long-term survival of AT-1 cardiomyocyte grafts in syngeneic myocardium. Am. J. Physiol. 1993. 264 (Heart Circ. Physiol. 33):H1727-H1733. Cited over 75 times*

Lash, J.A., Helper, D.J., Klug, M.G., Nicolozakes, A.W., and Hathaway, D.R. Nucleotide and deduced amino acid sequence of cDNA’s encoding two isoforms of the 17,000 dalton light chain in bovine aortic smooth muscle. Nucleic Acids Research. 1990. 18 (23):7176. Cited over 25 times*

* Citation numbers from ISI Web of Knowledge

REVIEW ARTICLES and BOOK CHAPTERS

Soonpaa, M.H., Klug, M.G., Nakajima, H., Nakajima, H. and Field, L.J. Potential approaches for cell-mediated myocardial repair. In Wilensky, R. (ed.), Unstable coronary artery disease: Pathology, diagnosis and treatment. 1998. Kluwer Academic Press, Boston, pp. 344-354.

Soonpaa, M.H., Daud, A.I., Koh, G.Y., Klug, M.G., Kim, K.K., Wang, H., and Field, L.J. Potential Approaches for Myocardial Regeneration. Annals New York Academy of Sciences. 1995. 752:446-454.

Klug, M.G., Daud, A.I., Chandrasekhar, S., and Field, L.J., Cardiogenesis from Commitment to the Adult Phenotype. In Zipes, D.P. and Jaliffe, J. (eds.) Cardiac Electrophysiology: from cell to bedside. 2nd ed. 1995. W.B. Saunders Comp. Philadelphia, pp. 57-63.

Koh, G.Y., Soonpaa, M.H., Klug, M.G., and Field, L.J. Strategies for Myocardial Repair. Journal of Interventional Cardiology. 1995. 8:387-393.

Koh, G.Y., Klug, M.G., and Field, L.J. Atrial Natriuretic Factor and Transgenic Mice. Hypertension. 1993. 22:634-639.

EXTERNAL PRESENTATIONS and CONFERENCES (selected)

MidAmerica Herbal Symposium, Altura, MN, September 15-17, 2017.

IDI, Intercultural Development Inventory, Qualified Administrator (QA), July 27-29, 2017.

MidAmerica Herbal Symposium, Altura, MN, September 16-18, 2016.

Medtronic, Fridley, MN, March 17, 2006. Invited presentation title: “Cardiomyocytes for cardiac engraftment and pharmaceutical discovery.”

Mayo Clinic, Rochester, MN, May 19, 2003. Invited presentation title: “Cardiac and skeletal muscle cultures for drug discovery.”

Klug, M.G. (presenting author) and Kube, D.M., Swiss Physiological Society, Obesity Conference. Institute of Physiology, University of Fribourg, Switzerland, September 21, 2001. Oral presentation: “Human Genome: what’s next for metabolic diseases.”

F. Hoffman-La Roche Ltd., Basel, Switzerland, May 20, 1999. Invited presentation title: “In vitro models for elucidating the pathophysiology of congestive heart failure.”

Advanced Cell Technology Inc., Worcester, MA, April 19, 1999. Invited presentation title: “Embryonic stem cell-derived cardiomyocyte grafts in adult mice.”

Geron Corporation, Menlo Park, CA, March 24, 1999. Invited presentation title: “Embryonic stem cell-derived cardiomyocytes for intra-cardiac grafting and drug discovery.”

Gordon Conference on Cardiac Regulatory Mechanisms, New London, New Hampshire, 1998. Poster title: “Different cardiomyocyte culture conditions influence markers utilized as in vitro surrogates of cardiac dysfunction.”

Institute for Human Gene Therapy, University of Pennsylvania, Philadelphia, Pennsylvania, February 27, 1998. Invited presentation title: “Muscle cell cultures and their applications.”

Lilly Research Laboratories, Indianapolis, IN, January 16, 1997. Invited presentation title: “Embryonic stem cell-derived cardiomyocyte cultures.”

Keystone Symposium on Tissue Engineering, Taos, New Mexico, 1996. Poster title: “Purification of cardiac myocytes differentiated from embryonic stem cells for use in intracardiac grafting.”

Keystone Symposium on Tissue Engineering, Taos, New Mexico, 1994. Poster title: “DNA synthesis and multinucleation in cardiomyocytes derived from embryonic stem cells.”

Keystone Symposium on the Developmental Biology of the Cardiovascular System, Taos, New Mexico, 1993. Poster title: “Cardiomyocyte development in embryoid bodies derived from murine embryonic stem cells.”

Gordon Conference on Developmental Biology of the Cardiovascular System, Tilton, New Hampshire, 1992. Competitive application to attend meeting accepted.

Gordon Conference on Cardiac Regulatory Mechanisms, Plymouth, New Hampshire, 1992. Competitive application to attend meeting accepted.

PUBLISHED ABSTRACTS

Klug, M.G., and Field, L.J., “DNA synthesis and multinucleation in cardiomyocytes derived from embryonic stem cells.” Keystone Symposium on Tissue Transplantation, Taos, New Mexico. [J. Cellular Biochemistry 18C, page 108, 1994]

Koh, G.Y., Kim, S.-J., Klug, M.G., Park, K., Soonpaa, M.H., Wang, H., and Field, L.J. “Local, long-term delivery of recombinant secretory TGF-b1 to the myocardium by using somatic gene transfer.” Keystone Symposium [J. Cellular Biochemistry 18A, page 238, 1994]

Klug, M.G., and Field, L.J., “Cardiomyocyte development in embryoid bodies derived from murine embryonic stem cells.” Keystone Symposium on the Developmental Biology of the Cardiovascular System, Taos, New Mexico [J. Cellular Biochemistry 17D, page 199, 1993]

Daud, A.I., Katz, E., Koh, G.Y., Klug, M.G., Soonpaa, M.H., Steinhelper, M.E., and Field, L.J. “Cardiomyocyte growth in transgenic animals.” Keystone Symposium on the Developmental Biology of the Cardiovascular System, Taos, New Mexico. [J. Cellular Biochemistry 17D, page 189, 1993]

Koh, G.Y., Soonpaa, M.H., Klug, M.G., and Field, L.J. “Long-term survival of AT-1 cardiomyocyte graft in syngeneic myocardium.” Keystone Symposium on the Developmental Biology of the Cardiovascular System, Taos, New Mexico. [J. Cellular Biochemistry 17D, page 199, 1993]

PATENTS

Quinoline Derivatives (US Patents 6,787,558; 7,012, 073; 7,064,134; 7,166,589 and US Patent Applications 20030158179, 20040259858, 20060063758, 20060148794). F. Hoffmann-La Roche Ltd., Basel, Switzerland.

Quinoline Derivatives as Neuropeptide Y Antagonists (WO03028726; Australian granted 2002342735, Korean granted 1020047004591, New Zealand granted 531517), F. Hoffmann-La Roche Ltd., Basel, Switzerland.

Excellent computer skills, including but not limited to: Microsoft Office (Word, PowerPoint, Excel, Outlook), Adobe Acrobat and Photoshop, Internet Explorer, Mozilla Thunderbird and Firefox, D2L (course delivery system), databases, & specialized software tools.

Art and Smart

March 14, 2018Leave a comment

Beloved Readers,

Art and Smart is a new magazine dedicated to the Art of Living Smart! Non-religious and spiritual, Art and Smart is full of interesting, valuable and entertaining information for you with no conflicts of interest. We worked hard for you, and we hope you enjoy reading Art and Smart and that it benefits you immensely!

Love, Mary and Michael

Copies can be purchased now on Amazon:

http://a.co/49hSME1

The front and back covers of the magazine (shown below) together make up one painting by ANT The Artist (the entirety of which is shown on page 15 of the magazine).

Marie Kube’s Curriculum Vitae

January 20, 2018March 14, 20182 Comments

Beloved Readers,

I am still trying to figure out how to make a living, in spite of all of my accomplishments! Here is my CV. Thank you for your consideration.

Lots of Love, Marie

Dr. Marie D. Kube, Ph.D.

2038 Ford Parkway, #369, St. Paul, MN, 55116

(currently residing in Las Vegas, NV)

http://www.myspiritualconnection.org/contact

Education

Ph.D., Dept. of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN, 1997, GPA 3.98/4.00

B.A., Chemistry (with minor in Mathematics), with distinction, University of Colorado at Denver, CO, 1990, GPA 3.87/4.00

Professional Experience

Author & Model 2011-Present

– Author, model, and photographer for Sexiest at 50: PTSD PhD Marie, edited by Dr. Michael G. Klug, Ph.D., a proven guide for healthy living to age 50, available on Amazon: http://a.co/3UJckBL

– Author of Instructions for Helping to Improve the Human Condition, a journey into the spiritual realm from a non-religious, scientifically trained perspective, available on Amazon: http://a.co/jeV4EB0

– Publisher of Art and Smart, a magazine dedicated to the Art of Living Smart, available now on Amazon: http://a.co/77FMdWs

– Creator of websites dedicated to the confluence of science and spirituality: www.myspiritualconnection.org, with at least 20 blog posts, and www.myspiritualassociation.com, with mature content and at least 25 blog posts

Volunteer

Minneapolis Community & Technical College (MCTC), Minneapolis, MN 2009-2016

– Assisted Dr. Michael G. Klug in developing and updating the curriculum and assessments, in grading, and in the preparations for his Molecular Biology BIOL 2500 course and laboratory that he taught Spring 2010, 2011, 2012, 2013, 2014, 2015, 2016

– Assisted Dr. Michael G. Klug with anything and everything I could do to promote success of students, colleagues, and MCTC

Consultant 2010-2011

University of Minnesota, Minneapolis, MN

– Consultant for development and commercialization of new technology

Director of Development 2007-2010

VitalMedix, Inc., Hudson, WI

– Developed a parenteral drug formulation for severe blood loss

– Planned and managed outsourcing of active pharmaceutical ingredient manufacturing, analytical assays, and pre-clinical safety & efficacy studies

– Coordinated with team members, external contractors and consultants for Pre-Investigational New Drug (Pre-IND) meeting with the FDA, including writing a well-received submission document with pre-clinical and clinical study plans

– Prepared and delivered technical presentations for current and potential investors and other stakeholders

– Wrote grant applications

Patent Agent (Reg. No. 59,744) 2005-2007

Fish & Richardson P.C., Minneapolis, MN

– Prepared and prosecuted numerous patent applications in the field of biotechnology

– Participated in patentability, due diligence, and freedom to operate analyses

Research Associate 2003-2005

Mayo Clinic, Rochester, MN

– Performed research for the prostate cancer biomarker discovery project of the Minnesota Partnership for Biotechnology and Medical Genomics

– Developed, optimized, and validated molecular biological methods

– Coordinated a multi-disciplinary team (including pathologists, bioinformaticians, molecular biologists, and protein biochemists) to achieve project goals within tight deadlines

– Generated, analyzed, presented, and published results

Contributing Editor 2002-2003

Reed Business Information, Morris Plains, NJ

– Published 16 articles about cutting edge technologies in Drug Discovery & Development and Genomics & Proteomics trade journals

– Interviewed high-level executives in small, medium, and large biotechnology and pharmaceutical companies

Laboratory Head and Project Leader 1999-2002

Hoffmann-La Roche, Basel, Switzerland

– Project leader of a drug discovery and development program, involving collaborations across departments and sites (Palo Alto, CA and Basel, Switzerland)

– Designed pre-clinical testing strategies

– Trained and supervised technicians

– Developed and validated in vitro assays

– Screened and characterized compounds

– Presented results to peers, management, and external scientific experts, advisors, and consultants

– Conducted research in obesity and type 2 diabetes as a postdoctoral researcher prior to being promoted

– Applied genomics (real-time, quantitative PCR and microarray technology) to study hypothalamic control of food intake in rodent models of food deprivation and overeating

– Inventor on U.S. Patent No. 6,900,227; U.S. Patent Application Publication No. 20050143373; and International Patent Application Publication No. WO2004013120

Instructor 1994-1996

Indiana University School of Medicine, Indianapolis, IN

– Facilitator for the Problem Based Learning Section of Medical Microbiology J601 for medical students (8/1996 to 12/1996)

– Instructor for Nursing Microbiology Lab J210 (1/1996 to 5/1996 and 8/1994 to 12/1994)

Associate Pharmaceutical Chemist 1990-1993

Lilly Research Laboratories, Eli Lilly and Co., Indianapolis, IN

– Developed the formulation for Humalog®

– Supported manufacture of clinical trial batches

– Provided supporting documentation for FDA approval

– Collaborated with statisticians to apply multivariate statistical methods to design experiments and analyze data

– Performed research on protein aggregation and protein crystal agglomeration in relation to physical stability of protein products

Honors and Awards

– Esther L. Kinsley Ph.D. Dissertation Award, Indiana University. This is the highest honor for research that Indiana University bestows upon its graduate students. Indiana University awarded 336 Ph.D.s in 1997 across all disciplines, and only one degree recipient was given the Kinsley Award.

– Travel Fellowship, Committee on Institutional Cooperation’s Women in Science and Engineering Initiative, 1997

– Sigma Xi Graduate Student Research Competition, Indiana University School of Medicine, Honorable Mention, 1996

– Travel Fellowship, Indiana University Purdue University at Indianapolis Fellowship Committee, 1995

– University Graduate Fellowship, Indiana University, 1994

– National Science Foundation Graduate Fellowship, Honorable Mention, 1994

– Outstanding Chemistry Student, The American Institute of Chemists Foundation, 1991

– Outstanding Undergraduate Student in the College of Liberal Arts and Sciences, University of Colorado at Denver, Finalist, 1991

– H.A. Arnold Scholarship, 1989-1990

– Colorado Dean’s List Scholarship, 1986-1987

– Regent’s Scholarship, 1985-1986

Publications (in chronological order)

A. Peer-Reviewed Journal Articles

Kube, D.M., and A. Srivastava. (1997) Quantitative DNA Slot Blot Analysis: Inhibition of DNA Binding to Membranes by Magnesium Ions. Nucleic Acids Res., 25:3375.

Kube, D.M., S. Ponnazhagan, and A. Srivastava. (1997) Encapsidation of Adeno-Associated Virus Type 2 Rep Proteins in Wild-Type and Recombinant Progeny Virions: Rep-Mediated Growth Inhibition of Primary Human Cells. J. Virol., 71:7361.

Qing, K., X.-S. Wang, D.M. Kube, S. Ponnazhagan, A. Bajpai, and A. Srivastava. (1997) Role of Tyrosine Phosphorylation of a Cellular Protein in Adeno-Associated Virus Type 2-Mediated Transgene Expression. Proc. Natl. Acad. Sci. USA, 94:10879.

Ponnazhagan, S., P. Mukherjee, X.-S. Wang, K. Qing, D.M. Kube, C. Mah, C. Kurpad, M.C. Yoder, E.F. Srour, and A. Srivastava. (1997) Adeno-Associated Virus Type 2-Mediated Transduction in Primary Human Bone Marrow-Derived CD34⁺ Hematopoietic Progenitor Cells: Donor Variation and Correlation of Transgene Expression with Cellular Differentiation. J. Virol., 71:8262.

Qing, K., B. Khuntirat, C. Mah, D.M. Kube, X.-S. Wang, S. Ponnazhagan, S. Zhou, V.J. Dwarki, M.C. Yoder, and A. Srivastava. (1998) Adeno-Associated Virus Type 2-Mediated Gene Transfer: Correlation of Tyrosine Phosphorylation of the Cellular Single-Stranded D Sequence-Binding Protein with Transgene Expression in Human Cells In Vitro and Murine Tissues In Vivo. J. Virol., 72:1593.

Wang, X.-S., B. Khuntirat, K. Qing, S. Ponnazhagan, D.M. Kube, S. Zhou, V.J. Dwarki, and A. Srivastava. (1998) Characterization of Wild-Type Adeno-Associated Virus Type 2-Like Particles Generated During Recombinant Viral Vector Production and Strategies for Their Elimination. J. Virol., 72:5472.

Mah, C., K. Qing, B. Khuntirat, S. Ponnazhagan, X.-S. Wang, D.M. Kube, M.C. Yoder, and A. Srivastava. (1998) Adeno-Associated Virus Type 2-Mediated Gene Transfer: Role of Epidermal Growth Factor Receptor Protein Tyrosine Kinase in Transgene Expression. J. Virol., 72:9835.

Rogers-Evans, M., A.I. Alanine, K.H. Bleicher, D. Kube, and G. Schneider. (2004) Identification of novel cannabinoid receptor ligands via evolutionary de novo design and rapid parallel synthesis. QSAR & Combinatorial Science, 23(6):426.

Kosari, F., A.S. Parker, D.M. Kube, C.M. Lohse, B.C. Leibovich, M.L. Blute, J.C. Cheville, and G. Vasmatzis. (2005) Clear Cell Renal Cell Carcinoma: Gene Expression Analyses Identify a Potential Signature for Tumor Aggressiveness. Clin. Cancer Res., 11:5128.

Kube, D.M., C.D. Savci-Heijink, A.-F. Lamblin, F. Kosari, G. Vasmatzis, J.C. Cheville, D.P. Connelly, and G.G. Klee. (2007) Optimization of Laser Capture Microdissection and RNA Amplification for Gene Expression Profiling of Prostate Cancer. BMC Molecular Biology, 8:25.

Vasmatzis, G., E. Klee, D.M. Kube, T. Therneau, and F. Kosari. (2007) Quantitating Tissue Specificity of Human Genes to Facilitate Biomarker Discovery. Bioinformatics, 23:1348.

Kosari, F., J.M.A. Munz, C.D. Savci-Heijink, C. Spiro, E.W. Klee, D.M. Kube, L. Tillmans, J. Slezak, R.J. Karnes, J.C. Cheville, and G. Vasmatzis. (2008) Identification of Prognostic Biomarkers for Prostate Cancer. Clin. Cancer Res., 14:1734.

Alig, L., J. Alsenz, M. Andjelkovic, S. Bendels, A. Bénardeau, K. Bleicher, A. Bourson, P. David-Pierson, W. Guba, S. Hildbrand, D. Kube, T. Lübbers, A. Mayweg, R. Narquizian, W. Neidhart, M. Nettekoven, J-.M. Plancher, C. Rocha, M. Rogers-Evans, S. Röver, G. Schneider, S. Taylor, and P. Waldmeier. (2008) Benzodioxoles: Novel Cannabinoid-1 Receptor Inverse Agonists for the Treatment of Obesity. J. Med. Chem., 51(7):2115.

B. Technical Reports

Kube, D.M. Chemical Genomics Energizes Discovery. Drug Discovery & Development, March 2002, Vol.5, No.3, p45.

Kube, D.M. Mining for Molecular Interactions. Genomics & Proteomics, April 2002.

Kube, D.M. Automatic DNA Purification and Sequencing Evolve. Drug Discovery & Development, July/August 2002, Vol.5, No.7, p38.

Kube, D.M. Genomics and Proteomics Continue to Pave the Way to the Clinic. Genomics & Proteomics, July/August 2002.

Kube, D.M. Multitalented Proteins Play a Key Role in Therapeutics. Genomics & Proteomics, September 2002.

Kube, D.M. A Model Approach to Streamline Drug Discovery. Drug Discovery & Development, October 2002, Vol.5, No.9, p59.

Kube, D.M. OLA, MDI, PEG: the latest words in drug delivery. Drug Discovery & Development, November 2002, Vol.5, No.10, p81.

Kube, D.M. Good Manufacturing Practice is Getting Better. Drug Discovery & Development, December 2002, Vol.5, No.11, p63.

Kube, D.M. Picking Potential Drugs with In Vitro ADMET Models. Drug Discovery & Development, January 2003, Vol.6, No.1, p50.

Kube, D.M. Metabonomics Treats Attrition in Early Drug Discovery. Drug Discovery & Development, March 2003, Vol.6, No.3, p45.

Kube, D.M. Profiles of Blockbuster Drugs and Effective Drug Targets. Drug Discovery & Development, March 2003, Vol.6, No.3, p73.

Kube, D.M. A Multidisciplinary Approach to Target Validation. Drug Discovery & Development, April 2003, Vol.6, No.4, p37.

Kube, D.M. Innovative Assay Platforms Drive Proteomics Research. Drug Discovery & Development, April 2003, Vol.6, No.4, p45.

Kube, D.M. Mass Spectrometry: Drug Discovery’s Essential Tool. Drug Discovery & Development, May 2003, Vol.6, No.5, p71.

Kube, D.M. Power of Automated Protein Fractionation. Genomics & Proteomics, May 2003, Vol.3, No.4, p36.

Kube, D.M. Rational Drug Design Enables Informed Decision-Making. Drug Discovery & Development, June 2003.

C. Presentations and Published Abstracts

Kim, Y., and D.M. Kube. (1991) Doppler Electrophoretic Light Scattering Analyzer for Determination of the Zeta Potential of Human Insulin Particles in Suspension. Lilly Research Labs Symposium, Indianapolis, IN.

Kube, D.M., S. Ponnazhagan, and A. Srivastava. (1995) AAV-Mediated Cytopathic Effect on Primary Human Bone Marrow Stromal Cells. VIth Parvovirus Workshop, Montpellier, France.

Srivastava, A., X.-S. Wang, S. Ponnazhagan, S.Z. Zhou, D.M. Kube, and M. Yoder. (1995) Parvovirus-Based Vectors for Human Gene Therapy. VIth Parvovirus Workshop, Montpellier, France.

Kube, D.M., S. Ponnazhagan, and A. Srivastava. (1995) Encapsidation of the Adeno-Associated Virus Type 2 Rep Proteins in Progeny Virions: Rep-Mediated Growth Inhibition of Primary Human Bone Marrow Stromal Cells. Blood, 86(10):3990.

Kube, D.M., S. Ponnazhagan, and A. Srivastava. (1997) Encapsidation of AAV Rep Proteins in Progeny Virions: Wild-Type and Recombinant AAV-Mediated Growth Inhibition of Primary Human Cells. International Parvovirus Workshop, Heidelberg, Germany.

Qing, K.Y., B. Khuntirat, C. Mah, D.M. Kube, X.-S. Wang, S. Ponnazhagan, S.Z. Zhou, V. Dwarki, M.C. Yoder, and A. Srivastava. (1997) Adeno-Associated Virus-Mediated Gene Transfer: Correlation of Tyrosine Phosphorylation with Transgene Expression in Human Cells In Vitro and Murine Tissues In Vivo. Blood, 90(10):525.

Ponnazhagan, S., P. Mukherjee, X.-S. Wang, K. Qing, D.M. Kube, C. Mah, C. Kurpad, M.C. Yoder, E.F. Srour, and A. Srivastava. (1997) AAV-Mediated Transduction of Primary Human Bone Marrow-Derived CD34+ Cells: Donor Variation and Correlation of Transgene Expression with Cellular Differentiation. International Parvovirus Workshop, Heidelberg, Germany.

Srivastava, A., S. Ponnazhagan, X.-S. Wang, K. Qing, P. Mukherjee, B. Khuntirat, D.M. Kube, C. Mah, and M.C. Yoder. (1997) AAV and Parvovirus B19 Vectors for Human Gene Therapy. International Parvovirus Workshop, Heidelberg, Germany.

Mah, C., K.Y. Qing, B. Khuntirat, S. Ponnazhagan, X.-S. Wang, D.M. Kube, M.C. Yoder, and A. Srivastava. (1998) Adeno-associated Virus 2-Mediated Gene Transfer: Role of Epidermal Growth Factor Receptor Protein Tyrosine Kinase in Transgene Expression. Blood, 92(10):150A.

Weigel, K.A., D.M. Kube, and A. Srivastava. (1998) Human Endothelial Cell-Specific Transgene Expression Mediated by a Recombinant Adeno-Associated Virus 2-Parvovirus B19 Hybrid Vector. Blood, 92(10):382B.

Kube, D.M., C.D. Savci-Heijink, A.-F. Lamblin, G. Vasmatzis, J.C. Cheville, D.P. Connelly, and G.G. Klee. (2005) Optimization of Laser Capture Microdissection and RNA Amplification for Gene Expression Profiling of Prostate Cancer. American Association for Cancer Research, Anaheim, CA.

D. Patents

– Benzodioxole Derivatives, U.S. Patent No. 6,900,227, U.S. Patent No. 7,576,088, Hoffman-La Roche.

– Novel Benzodioxoles, International Patent Application Publication No. WO2004013120 (granted in Australia, European Patent Office, India, Republic of Korea and New Zealand), European Patent Specification No. EP 1 532 132, F. Hoffman-La Roche.

WHERE HAVE ALL THE FLOWERS GONE?

October 8, 2017Leave a comment

This is the first piece of writing I submitted for consideration to someone who was not one of my teachers. Rather, it was the essay portion of a scholarship I competed for when I finished high school and was about to start college. It was 1985. I was 18 years old. The title, “Where have all the flowers gone?” was given as the prompt for the essay, and you were supposed to write an essay to go with that title. Here’s mine. I used a typewriter to produce the original version (see below) before we even had word processors, let alone personal computers! I was rejected. It is said that the job of an artist is to accurately reflect the times…

WHERE HAVE ALL THE FLOWERS GONE?

Throughout literature children have been symbolized as flowers. The comparison does not hold true as time passes, however, because children from generation to generation are so distinct. In fact, saintly children from the past worthy of being symbolized as flowers are scarce if not extinct in the modern world. The reason for the dwindling amount of flowers is the way in which society is changing in attitude and action according to the technological advances being made. The close-knit family unit has disintegrated since women have etched their own space in the man’s world. Now computers and tape recorders read to children in place of parents, thereby causing neglect of the love and respect factor. Children are not taught to love and respect because they are raised by friends and babysitters. The increased amount of knowledge available makes kids feel smarter than their elders. They believe knowledge replaces experience and is more important. They fail to realize that experience and not book knowledge is the true teacher. Therefore, respect for elders is lost. Because of the pressures and stress of the fast-paced lifestyle necessary to survive in today’s society, little things like fishing with grandpa have little or no value anymore. People are more concerned with how many errands they can run in one day and how much money they can earn than with planning a family picnic. Materialism is obviously overpowering humanism and appreciation for nature. Such a materialistic outlook can only lead to immorality because materialism completely deviates from the principles of the bible which are the only basis of right and wrong conduct that exists. Since religion is being replaced by science and the need for God by money, moral standards are being diluted. Modern-day children lack appreciation and have only expectations. Modern conveniences like dishwashers, microwave ovens, and cars facilitate life. Walking two miles to school through rain and snow and doing three hours of chores before breakfast are unheard of today. Since things come too easily to today’s children, they are much more improvident, squandering, and wasteful. As a painful but honest look reveals, the flowers are fading, wilting, and drying up through the generations.

I Can’t Believe I Published Another Book

September 13, 2017September 13, 2017Leave a comment

Most Blessed Reader,

These Instructions for Helping to Improve the Human Condition were born out of severe and prolonged pain and suffering. I had to find a way to live or else die. It became ever clearer to me that our Lord is the only source of comfort. I became extremely proficient at achieving an altered state of mind that allows me to escape to heaven at will and whenever necessary to conquer the hell on earth.

I and my works have been largely oppressed, denied, and rejected. Most everyone else believes that they are more important and have suffered more than me. Furthermore, if they looked as good as I do, they certainly would not suffer. That is because they love power and supremacy and would use my gifts in attempt to satisfy their cravings to control others and excel in the kind of glory fabricated out of money in this material world.

Don’t compare yourself to me because I, by immaculate conception, have the advantage of being free of original sin, and I am the closest with the Lord. I am Mary, the mother of God, the woman of the Apocalypse, a scientist and a writer. A mother knows her child better than anyone else. My Child is coming back to bring heaven to earth and fulfill the Lord’s prayer from beginning to end.

The best thing that ever happened to me was meeting Dr. Michael G. Klug, Ph.D., the Archangel Michael. I had waited so long for him and searched so hard for him. Our love was so great and we worked together so well that we were relentlessly attacked by the devils and satans so full of love for themselves and the material world and so prideful in their own intelligence. Too much ugliness got between us for our marriage to survive. But all the evil in this world cannot destroy love and truth, and our love is true. Some say that love equals respect. Our mutual respect combined with our mutual and primary goal of making this world a better place survives stronger than ever, allowing us to continue working together always even better than before as understanding grows.

During my involuntary unemployment and his involuntary employment, Michael supported me just like in the picture of us in Juan Diego’s cloak from almost 500 years ago that is now hanging in the Basilica of Our Lady of Guadalupe in the north of Mexico City. I researched, read, studied, and wrote for years. I read and studied the Word. I paid professionals to hypnotize me and teach me spirit releasements. I read their books and their predecessors’ books. I learned that 90% of societies worldwide have records of spirit attachment phenomena. I figured out how to share my experience to help others achieve what I have achieved. Michael remains the best reader I ever met. He is my first reader, my highest standard.

I finished writing these Instructions for Helping to Improve the Human Condition about four years ago, and Michael and I have used them extensively to better handle personal losses and traumas, deaths of loved ones, even my PTSD. The journey for both of us has involved extensive self-discovery and personal growth. We have gotten better and our lives have gotten better. Therefore, we want to share what we have learned with you. What else would Mary want but for you to know her Child? For the idea of God enters all religion and affects conjunction with God, and by conjunction, salvation.

I offer all this information and more for free on my websites. I am publishing this book to empower more people, especially in the aftermath of the devastation of Hurricane Harvey, which was previewed to us when we were using these Instructions for Helping to Improve the Human Condition months prior to the event, as documented on the websites. This is the ultimate self-help method best done with a friend as it can involve hypnosis.

Love,

Mary

You can buy the book at amazon.com: http://a.co/77UxP8a

I Can’t Believe I Published a Book

August 25, 2017Leave a comment

Dear Friend,

It has been an honor serving the Lord with the Archangel Michael for more than 20 years, and I had fun making a book with him in 4 cities in 3 states of the continental U.S. Who knew that he is a gifted photographer, too? I know that the foreword he wrote will melt the heart of anyone who reads it. I don’t know how to thank him except by contributing some beauty to the world and, most importantly, love.

We published the book on August 21, 2017, after a total solar eclipse happened. I couldn’t see the eclipse for myself, even though I bought safety viewing glasses. It rained in the desert that day. The sun usually returns quickly after a rain in the desert, especially during August, but on the 21^st it disappeared shortly after appearing and a thick cloud cover persisted even beyond the potential viewing period. The unexpected sign I saw that day was this double rainbow:

Since its release, “Sexiest at 50: PTSD PhD Marie” has been the #1 New Release in Erotic Arts & Photography on amazon.com. I am very honored and very grateful!

The course I took on Pornographic and Erotic Literature at CU Denver during the spring semester of 1990 sure paid off!

I did not think that I needed a liberal arts education as a chemistry major. But my professors assured me that it was necessary for me to become “well-rounded” and “to have something to talk with people about at dinner parties.” I ended up talking about that course in Pornographic and Erotic Literature years later with the head of the department I was interviewing in for graduate school. He asked me about the anomaly on my transcript. I thought he must be talking about the C⁺ I received in physics. I proceeded to explain that I had the third highest grade in a class of like 160 students. I went to meet with the professor in person to get the error corrected, but he told me that he gave one A, one B, one C⁺, and the rest C’s, D’s, and F’s. He assured me that he could do whatever he wanted to, and I believed him.

In fact, my future department head could not care less about physics. What he wanted to know about was erotic and pornographic literature. Turns out most everyone is more interested in erotic and pornographic literature than in physics.

By the time I finished my bachelor’s degree, I was so broke that I had to get a credit card just to buy clothes suitable for job interviews. I protested, but my professors assured me that it was necessary. They really did an excellent job educating me at CU Denver. My clothes were torn and tattered, as is fashionable these days! People actually buy clothes that have been purposely torn and faded to imitate how I looked when I was really poor and my car barely ran. That’s okay because I learned how to live economically, which is good because I must sell like 8 books every day just to pay $700/mo rent.

Publish or perish…♡

Thank God for believers, the sexiest people in the world!

Love,

Mary

You can get a copy of the book here: https://www.amazon.com/dp/1974551415/ref=cm_sw_r_cp_awdb_P8yNzb3X1E93Q

Vegas Murals

July 18, 20172 Comments

11 favorite Vegas murals

Email to the President of Minneapolis Community & Technical College regarding Student Success

July 2, 2017Leave a comment

Letter to MCTC President re student success jpeg.jpg

Vegas Vive

June 27, 2017June 27, 2017Leave a comment

Dear Readers,

My current apartment is about the same size as the first apartment I ever rented by myself when I was working my way through college, a one-bedroom apartment.

It still looks the same, even though the roof was leaking into my third-floor apartment back then, thirty years ago.

Nowadays, I am not accepted as an applicant on my own, even though I have an excellent credit score and way more money, accomplishments, and knowledge than I had three decades ago. My ex-husband, bless his heart, signed the lease so I could live here. I, along with so many other PhD-level scientists, have been long-term unemployed and shamed about it as if it is our fault that greed and corruption took over. Meanwhile, everyone thinks I am privileged because I am white and I look good. But I do not look good enough to rent an apartment, even though I have the money. “You can’t judge a book by its cover,” they say, but people do anyway.

For we wrestle not against flesh and blood, but against principalities, against powers, against the rulers of the darkness of this world, against spiritual wickedness in high places (Ephesians 6:12 KJV).

I have lived in Canada (where I was born), New Mexico, Colorado, Indiana, Pennsylvania, Switzerland, and Minnesota. I have visited even more places. Now I am moving to Nevada. In Las Vegas, more than anywhere else I have lived, I could go from tourist to local in one day, like a fish taking to water, only harsh, scorching hot desert. It is a doctor’s dream come true, there is so much opportunity to help people here. Why, one can even save lives.

Divine providence of the Lord prepared a place for me in the wilderness, indeed. It is where I am most comfortable, with the oppressed, the impoverished, the desperate, the pariahs. I see them and they see me. I am home. I finally sleep. The excessive heat leaves me no other choice anyway.

I avoid the most sizzling of the sun’s rays like a vampire. I wear sunscreen, hats, and sunglasses, and I drink lots of water. I condition my hair much more than shampoo it. I moisturize my skin with organic jojoba oil, actually a wax, that is most like the oils naturally produced by our skin.

Making money and having money are two different things. I never got any handouts or assistance; I earned everything that I have despite violent opposition. I saved money by always living below my means, even during the times when I made a lot of money. I work hard and smart. I do not waste money. I hardly got any retirement income during my career. I always made less than the men.

I occupy the same size space that I did thirty years ago, but my collection of stuff is infinitely more refined and complicated in reflection of my work and abilities. I did not have children of my own, which allowed me to be always generous towards others. I have most of the one bedroom empty so I can do yoga and dance there. My bed is in the living room. When I arrived in Vegas, all my bank accounts were put on hold. I had to straighten it all out.

Most importantly, I can finally preach the truth, free from any conflict of interest. And since I know what the truth is, that makes me simultaneously the most powerful and endangered woman on earth.

Whosoever will come after me, let him deny himself, and take up his cross, and follow me. For whosoever will save his life shall lose it; but whosoever shall lose his life for my sake and the gospel’s, the same shall save it (Mark 8:34-35 KJV).

In the last neighborhood I lived in, gentrification removed the cock. But not here, thankfully. My neighbor is a real cock.

Blessed are the meek: for they shall inherit the earth (Matthew 5:5 KJV).