The Book Beat Bookstore

The Book Beat bookstore has Marie Kube’s books!

Dearest Readers,

The Book Beat bookstore has our books for sale now! Open since 1982, the Book Beat offers valuable African art along with the most progressive, forward-thinking publications. Please stop by the Book Beat and look for Marie Kube’s books in the local authors’ section under “K” for Kube. Our goal is to offer our books for sale at bookstores for the same price or much less than on amazon.com online. This is because I have a special understanding of the need for independent bookstores since my grandfather owned a bookstore in Berlin, Germany that was destroyed in World War II.

Please visit the Book Beat at:
26010 Greenfield Rd.
Oak Park, MI 48237
248 / 968-1190
http://www.thebookbeat.com

“An independent bookstore specializing in art, photography, & children’s books”

And if you are in the Chicago, Illinois area, then you can also find our books for sale at Quimby’s Bookstore (click here for more information).

Thank you for your support!

Love,
Marie

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Art and Smart

Beloved Readers,

Art and Smart is a new magazine dedicated to the Art of Living Smart!  Non-religious and spiritual, Art and Smart is full of interesting, valuable and entertaining information for you with no conflicts of interest.  We worked hard for you, and we hope you enjoy reading Art and Smart and that it benefits you immensely!

Love, Mary and Michael

Copies can be purchased now on Amazon:

http://a.co/49hSME1

The front and back covers of the magazine (shown below) together make up one painting by ANT The Artist (the entirety of which is shown on page 15 of the magazine).

front scan

back scan

 

 

Marie Kube’s Curriculum Vitae

Beloved Readers,

I am still trying to figure out how to make a living, despite all of my accomplishments!  Here is my CV.  Thank you for your consideration.

Lots of Love, Marie

Dr. Marie D. Kube, Ph.D.

2038 Ford Parkway, #369, St. Paul, MN, 55116

(currently residing in Las Vegas, NV)

http://www.art-and-smart.com/contact

Education

Ph.D., Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN, 1997, GPA 3.98/4.00

B.A., Chemistry (with minor in Mathematics), with distinction, University of Colorado at Denver, CO, 1990, GPA 3.87/4.00

Professional Experience

Author & Model                                                                                                            2011-Present

–        Author, model, and photographer for Sexiest at 50: PTSD PhD Marie, edited by Dr. Michael G. Klug, Ph.D., a proven guide for healthy living to age 50, available here

–        Author of Instructions for Helping to Improve the Human Condition, a journey into the spiritual realm from a non-religious, scientifically trained perspective, available here and here

–        Publisher of Art and Smart, edited by Dr. Michael G. Klug, Ph.D., a magazine dedicated to the Art of Living Smart, now available here

–        Creator of websites dedicated to the confluence of science and spirituality:  www.art-and-smart.com, with at least 20 blog posts, and www.myspiritualassociation.com, with mature content and at least 25 blog posts

Volunteer                                                                                                                             2009-2016

Minneapolis Community & Technical College (MCTC), Minneapolis, MN

–        Assisted Dr. Michael G. Klug in developing and updating the curriculum and assessments, in grading, and in the preparations for his Molecular Biology BIOL 2500 course and laboratory that he taught Spring 2010, 2011, 2012, 2013, 2014, 2015, 2016

–        Assisted Dr. Michael G. Klug with anything and everything I could do to promote success of students, colleagues, and MCTC

 Consultant                                                                                                                          2010-2011

University of Minnesota, Minneapolis, MN

–        Consultant for development and commercialization of new technology

Director of Development                                                                                                2007-2010

VitalMedix, Inc., Hudson, WI

–        Developed a parenteral drug formulation for severe blood loss

–        Planned and managed outsourcing of active pharmaceutical ingredient manufacturing, analytical assays, and pre-clinical safety & efficacy studies

–        Coordinated with team members, external contractors and consultants for Pre-Investigational New Drug (Pre-IND) meeting with the FDA, including writing a well-received submission document with pre-clinical and clinical study plans

–        Prepared and delivered technical presentations for current and potential investors and other stakeholders

–        Wrote grant applications

Patent Agent (Reg. No. 59,744)                                                                                       2005-2007

Fish & Richardson P.C., Minneapolis, MN

–        Prepared and prosecuted numerous patent applications in the field of biotechnology

–        Participated in patentability, due diligence, and freedom to operate analyses

Research Associate                                                                                                           2003-2005

Mayo Clinic, Rochester, MN

–        Performed research for the prostate cancer biomarker discovery project of the Minnesota Partnership for Biotechnology and Medical Genomics

–        Developed, optimized, and validated molecular biological methods

–        Coordinated a multi-disciplinary team (including pathologists, bioinformaticians, molecular biologists, and protein biochemists) to achieve project goals within tight deadlines

–        Generated, analyzed, presented, and published results

Contributing Editor                                                                                                          2002-2003

Reed Business Information, Morris Plains, NJ

–        Published 16 articles about cutting edge technologies in Drug Discovery & Development and Genomics & Proteomics trade journals

–        Interviewed high-level executives in small, medium, and large biotechnology and pharmaceutical companies

 Laboratory Head and Project Leader                                                                        1999-2002

Hoffmann-La Roche, Basel, Switzerland

–        Project leader of a drug discovery and development program, involving collaborations across departments and sites (Palo Alto, CA and Basel, Switzerland)

–        Designed pre-clinical testing strategies

–        Trained and supervised technicians

–        Developed and validated in vitro assays

–        Screened and characterized compounds

–        Presented results to peers, management, and external scientific experts, advisors, and consultants

–        Conducted research in obesity and type 2 diabetes as a postdoctoral researcher prior to being promoted

–        Applied genomics (real-time, quantitative PCR and microarray technology) to study hypothalamic control of food intake in rodent models of food deprivation and overeating

–        Inventor on U.S. Patent No. 6,900,227; U.S. Patent Application Publication No. 20050143373; and International Patent Application Publication No. WO2004013120

Instructor                                                                                                                            1994-1996

Indiana University School of Medicine, Indianapolis, IN

– Facilitator for the Problem Based Learning Section of Medical Microbiology J601 for medical students (8/1996 to 12/1996)

– Instructor for Nursing Microbiology Lab J210 (1/1996 to 5/1996 and 8/1994 to 12/1994)

Associate Pharmaceutical Chemist                                                                             1990-1993

Lilly Research Laboratories, Eli Lilly and Co., Indianapolis, IN

–        Developed the formulation for Humalog®

–        Supported manufacture of clinical trial batches

–        Provided supporting documentation for FDA approval

–        Collaborated with statisticians to apply multivariate statistical methods to design experiments and analyze data

–        Performed research on protein aggregation and protein crystal agglomeration in relation to physical stability of protein products

Honors and Awards

– Esther L. Kinsley Ph.D. Dissertation Award, Indiana University.  This is the highest honor for research that Indiana University bestows upon its graduate students.  Indiana University awarded 336 Ph.D.s in 1997 across all disciplines, and only one degree recipient was given the Kinsley Award.

– Travel Fellowship, Committee on Institutional Cooperation’s Women in Science and Engineering Initiative, 1997

– Sigma Xi Graduate Student Research Competition, Indiana University School of Medicine, Honorable Mention, 1996

– Travel Fellowship, Indiana University Purdue University at Indianapolis Fellowship Committee, 1995

– University Graduate Fellowship, Indiana University, 1994

– National Science Foundation Graduate Fellowship, Honorable Mention, 1994

– Outstanding Chemistry Student, The American Institute of Chemists Foundation, 1991

– Outstanding Undergraduate Student in the College of Liberal Arts and Sciences, University of Colorado at Denver, Finalist, 1991

– H.A. Arnold Scholarship, 1989-1990

– Colorado Dean’s List Scholarship, 1986-1987

– Regent’s Scholarship, 1985-1986

Publications (in chronological order)

A. Peer-Reviewed Journal Articles

Kube, D.M., and A. Srivastava. (1997) Quantitative DNA Slot Blot Analysis: Inhibition of DNA Binding to Membranes by Magnesium Ions. Nucleic Acids Res., 25:3375.

Kube, D.M., S. Ponnazhagan, and A. Srivastava. (1997) Encapsidation of Adeno-Associated Virus Type 2 Rep Proteins in Wild-Type and Recombinant Progeny Virions: Rep-Mediated Growth Inhibition of Primary Human Cells. J. Virol., 71:7361.

Qing, K., X.-S. Wang, D.M. Kube, S. Ponnazhagan, A. Bajpai, and A. Srivastava. (1997) Role of Tyrosine Phosphorylation of a Cellular Protein in Adeno-Associated Virus Type 2-Mediated Transgene Expression. Proc. Natl. Acad. Sci. USA, 94:10879.

Ponnazhagan, S., P. Mukherjee, X.-S. Wang, K. Qing, D.M. Kube, C. Mah, C. Kurpad, M.C. Yoder, E.F. Srour, and A. Srivastava. (1997) Adeno-Associated Virus Type 2-Mediated Transduction in Primary Human Bone Marrow-Derived CD34+ Hematopoietic Progenitor Cells: Donor Variation and Correlation of Transgene Expression with Cellular Differentiation. J. Virol., 71:8262.

Qing, K., B. Khuntirat, C. Mah, D.M. Kube, X.-S. Wang, S. Ponnazhagan, S. Zhou, V.J. Dwarki, M.C. Yoder, and A. Srivastava. (1998) Adeno-Associated Virus Type 2-Mediated Gene Transfer: Correlation of Tyrosine Phosphorylation of the Cellular Single-Stranded D Sequence-Binding Protein with Transgene Expression in Human Cells In Vitro and Murine Tissues In Vivo. J. Virol., 72:1593.

Wang, X.-S., B. Khuntirat, K. Qing, S. Ponnazhagan, D.M. Kube, S. Zhou, V.J. Dwarki, and A. Srivastava. (1998) Characterization of Wild-Type Adeno-Associated Virus Type 2-Like Particles Generated During Recombinant Viral Vector Production and Strategies for Their Elimination. J. Virol., 72:5472.

Mah, C., K. Qing, B. Khuntirat, S. Ponnazhagan, X.-S. Wang, D.M. Kube, M.C. Yoder, and A. Srivastava. (1998) Adeno-Associated Virus Type 2-Mediated Gene Transfer: Role of Epidermal Growth Factor Receptor Protein Tyrosine Kinase in Transgene Expression. J. Virol., 72:9835.

Rogers-Evans, M., A.I. Alanine, K.H. Bleicher, D. Kube, and G. Schneider. (2004) Identification of novel cannabinoid receptor ligands via evolutionary de novo design and rapid parallel synthesis. QSAR & Combinatorial Science, 23(6):426.

Kosari, F., A.S. Parker, D.M. Kube, C.M. Lohse, B.C. Leibovich, M.L. Blute, J.C. Cheville, and G. Vasmatzis. (2005) Clear Cell Renal Cell Carcinoma: Gene Expression Analyses Identify a Potential Signature for Tumor Aggressiveness. Clin. Cancer Res., 11:5128.

Kube, D.M., C.D. Savci-Heijink, A.-F. Lamblin, F. Kosari, G. Vasmatzis, J.C. Cheville, D.P. Connelly, and G.G. Klee. (2007) Optimization of Laser Capture Microdissection and RNA Amplification for Gene Expression Profiling of Prostate Cancer. BMC Molecular Biology, 8:25.

Vasmatzis, G., E. Klee, D.M. Kube, T. Therneau, and F. Kosari. (2007) Quantitating Tissue Specificity of Human Genes to Facilitate Biomarker Discovery.  Bioinformatics, 23:1348.

Kosari, F., J.M.A. Munz, C.D. Savci-Heijink, C. Spiro, E.W. Klee, D.M. Kube, L. Tillmans, J. Slezak, R.J. Karnes, J.C. Cheville, and G. Vasmatzis. (2008) Identification of Prognostic Biomarkers for Prostate Cancer. Clin. Cancer Res., 14:1734.

Alig, L., J. Alsenz, M. Andjelkovic, S. Bendels, A. Bénardeau, K. Bleicher, A. Bourson, P. David-Pierson, W. Guba, S. Hildbrand, D. Kube, T. Lübbers, A. Mayweg, R. Narquizian, W. Neidhart, M. Nettekoven, J-.M. Plancher, C. Rocha, M. Rogers-Evans, S. Röver, G. Schneider, S. Taylor, and P. Waldmeier. (2008) Benzodioxoles:  Novel Cannabinoid-1 Receptor Inverse Agonists for the Treatment of Obesity.  J. Med. Chem., 51(7):2115.

 B. Technical Reports

Kube, D.M. Chemical Genomics Energizes Discovery. Drug Discovery & Development, March 2002, Vol.5, No.3, p45.

Kube, D.M. Mining for Molecular Interactions. Genomics & Proteomics, April 2002.

Kube, D.M. Automatic DNA Purification and Sequencing Evolve. Drug Discovery & Development, July/August 2002, Vol.5, No.7, p38.

Kube, D.M. Genomics and Proteomics Continue to Pave the Way to the Clinic. Genomics & Proteomics, July/August 2002.

Kube, D.M. Multitalented Proteins Play a Key Role in Therapeutics. Genomics & Proteomics, September 2002.

Kube, D.M. A Model Approach to Streamline Drug Discovery. Drug Discovery & Development, October 2002, Vol.5, No.9, p59.

Kube, D.M. OLA, MDI, PEG: the latest words in drug delivery. Drug Discovery & Development, November 2002, Vol.5, No.10, p81.

Kube, D.M. Good Manufacturing Practice is Getting Better. Drug Discovery & Development, December 2002, Vol.5, No.11, p63.

Kube, D.M. Picking Potential Drugs with In Vitro ADMET Models. Drug Discovery & Development, January 2003, Vol.6, No.1, p50.

Kube, D.M. Metabonomics Treats Attrition in Early Drug Discovery. Drug Discovery & Development, March 2003, Vol.6, No.3, p45.

Kube, D.M. Profiles of Blockbuster Drugs and Effective Drug Targets. Drug Discovery & Development, March 2003, Vol.6, No.3, p73.

Kube, D.M. A Multidisciplinary Approach to Target Validation. Drug Discovery & Development, April 2003, Vol.6, No.4, p37.

Kube, D.M. Innovative Assay Platforms Drive Proteomics Research. Drug Discovery & Development, April 2003, Vol.6, No.4, p45.

Kube, D.M. Mass Spectrometry: Drug Discovery’s Essential Tool. Drug Discovery & Development, May 2003, Vol.6, No.5, p71.

Kube, D.M. Power of Automated Protein Fractionation. Genomics & Proteomics, May 2003, Vol.3, No.4, p36.

Kube, D.M. Rational Drug Design Enables Informed Decision-Making. Drug Discovery & Development, June 2003.

C. Presentations and Published Abstracts

Kim, Y., and D.M. Kube. (1991) Doppler Electrophoretic Light Scattering Analyzer for Determination of the Zeta Potential of Human Insulin Particles in Suspension. Lilly Research Labs Symposium, Indianapolis, IN.

Kube, D.M., S. Ponnazhagan, and A. Srivastava. (1995) AAV-Mediated Cytopathic Effect on Primary Human Bone Marrow Stromal Cells. VIth Parvovirus Workshop, Montpellier, France.

Srivastava, A., X.-S. Wang, S. Ponnazhagan, S.Z. Zhou, D.M. Kube, and M. Yoder. (1995) Parvovirus-Based Vectors for Human Gene Therapy. VIth Parvovirus Workshop, Montpellier, France.

Kube, D.M., S. Ponnazhagan, and A. Srivastava. (1995) Encapsidation of the Adeno-Associated Virus Type 2 Rep Proteins in Progeny Virions: Rep-Mediated Growth Inhibition of Primary Human Bone Marrow Stromal Cells. Blood, 86(10):3990.

Kube, D.M., S. Ponnazhagan, and A. Srivastava. (1997) Encapsidation of AAV Rep Proteins in Progeny Virions: Wild-Type and Recombinant AAV-Mediated Growth Inhibition of Primary Human Cells. International Parvovirus Workshop, Heidelberg, Germany.

Qing, K.Y., B. Khuntirat, C. Mah, D.M. Kube, X.-S. Wang, S. Ponnazhagan, S.Z. Zhou, V. Dwarki, M.C. Yoder, and A. Srivastava. (1997) Adeno-Associated Virus-Mediated Gene Transfer: Correlation of Tyrosine Phosphorylation with Transgene Expression in Human Cells In Vitro and Murine Tissues In Vivo. Blood, 90(10):525.

Qing, K., X.-S. Wang, D.M. Kube, S. Ponnazhagan, A. Bajpai, and A. Srivastava. (1997) Role of Tyrosine Phosphorylation of a Cellular Protein in Adeno-Associated Virus Type 2-Mediated Transgene Expression. International Parvovirus Workshop, Heidelberg, Germany. (Blood, 90(10):526).

Ponnazhagan, S., P. Mukherjee, X.-S. Wang, K. Qing, D.M. Kube, C. Mah, C. Kurpad, M.C. Yoder, E.F. Srour, and A. Srivastava. (1997) AAV-Mediated Transduction of Primary Human Bone Marrow-Derived CD34+ Cells: Donor Variation and Correlation of Transgene Expression with Cellular Differentiation. International Parvovirus Workshop, Heidelberg, Germany.

Srivastava, A., S. Ponnazhagan, X.-S. Wang, K. Qing, P. Mukherjee, B. Khuntirat, D.M. Kube, C. Mah, and M.C. Yoder. (1997) AAV and Parvovirus B19 Vectors for Human Gene Therapy. International Parvovirus Workshop, Heidelberg, Germany.

Mah, C., K.Y. Qing, B. Khuntirat, S. Ponnazhagan, X.-S. Wang, D.M. Kube, M.C. Yoder, and A. Srivastava. (1998) Adeno-associated Virus 2-Mediated Gene Transfer: Role of Epidermal Growth Factor Receptor Protein Tyrosine Kinase in Transgene Expression. Blood, 92(10):150A.

Weigel, K.A., D.M. Kube, and A. Srivastava. (1998) Human Endothelial Cell-Specific Transgene Expression Mediated by a Recombinant Adeno-Associated Virus 2-Parvovirus B19 Hybrid Vector. Blood, 92(10):382B.

Kube, D.M., C.D. Savci-Heijink, A.-F. Lamblin, G. Vasmatzis, J.C. Cheville, D.P. Connelly, and G.G. Klee. (2005) Optimization of Laser Capture Microdissection and RNA Amplification for Gene Expression Profiling of Prostate Cancer. American Association for Cancer Research, Anaheim, CA.

D. Patents

– Benzodioxole Derivatives, U.S. Patent No. 6,900,227, U.S. Patent No. 7,576,088, Hoffman-La Roche.

– Novel Benzodioxoles, International Patent Application Publication No. WO2004013120 (granted in Australia, European Patent Office, India, Republic of Korea and New Zealand), European Patent Specification No. EP 1 532 132, F. Hoffman-La Roche.

 

WHERE HAVE ALL THE FLOWERS GONE?

This is the first piece of writing I submitted for consideration to someone who was not one of my teachers.  Rather, it was the essay portion of a scholarship I competed for when I finished high school and was about to start college.  It was 1985.  I was 18 years old.  The title, “Where have all the flowers gone?” was given as the prompt for the essay, and you were supposed to write an essay to go with that title.  Here’s mine.  I used a typewriter to produce the original version (see below) before we even had word processors, let alone personal computers!  I was rejected.  It is said that the job of an artist is to accurately reflect the times…

WHERE HAVE ALL THE FLOWERS GONE?

Throughout literature children have been symbolized as flowers.  The comparison does not hold true as time passes, however, because children from generation to generation are so distinct.  In fact, saintly children from the past worthy of being symbolized as flowers are scarce if not extinct in the modern world.  The reason for the dwindling amount of flowers is the way in which society is changing in attitude and action according to the technological advances being made.  The close-knit family unit has disintegrated since women have etched their own space in the man’s world.  Now computers and tape recorders read to children in place of parents, thereby causing neglect of the love and respect factor.  Children are not taught to love and respect because they are raised by friends and babysitters.  The increased amount of knowledge available makes kids feel smarter than their elders.  They believe knowledge replaces experience and is more important.  They fail to realize that experience and not book knowledge is the true teacher.  Therefore, respect for elders is lost.  Because of the pressures and stress of the fast-paced lifestyle necessary to survive in today’s society, little things like fishing with grandpa have little or no value anymore.  People are more concerned with how many errands they can run in one day and how much money they can earn than with planning a family picnic.  Materialism is obviously overpowering humanism and appreciation for nature.  Such a materialistic outlook can only lead to immorality because materialism completely deviates from the principles of the bible which are the only basis of right and wrong conduct that exists.  Since religion is being replaced by science and the need for God by money, moral standards are being diluted.  Modern-day children lack appreciation and have only expectations.  Modern conveniences like dishwashers, microwave ovens, and cars facilitate life.  Walking two miles to school through rain and snow and doing three hours of chores before breakfast are unheard of today.  Since things come too easily to today’s children, they are much more improvident, squandering, and wasteful.  As a painful but honest look reveals, the flowers are fading, wilting, and drying up through the generations.

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I Can’t Believe I Published Another Book

Most Blessed Reader,

These Instructions for Helping to Improve the Human Condition were born out of severe and prolonged pain and suffering.  I had to find a way to live or else die.  It became ever clearer to me that our Lord is the only source of comfort.  I became extremely proficient at achieving an altered state of mind that allows me to escape to heaven at will and whenever necessary to conquer the hell on earth.

I and my works have been largely oppressed, denied, and rejected.  Most everyone else believes that they are more important and have suffered more than me.  Furthermore, if they looked as good as I do, they certainly would not suffer.  That is because they love power and supremacy and would use my gifts in attempt to satisfy their cravings to control others and excel in the kind of glory fabricated out of money in this material world.

Don’t compare yourself to me because I, by immaculate conception, have the advantage of being free of original sin, and I am the closest with the Lord.  I am Mary, the mother of God, the woman of the Apocalypse, a scientist and a writer.  A mother knows her child better than anyone else.  My Child is coming back to bring heaven to earth and fulfill the Lord’s prayer from beginning to end.

The best thing that ever happened to me was meeting Dr. Michael G. Klug, Ph.D., the Archangel Michael.  I had waited so long for him and searched so hard for him.  Our love was so great and we worked together so well that we were relentlessly attacked by the devils and satans so full of love for themselves and the material world and so prideful in their own intelligence.  Too much ugliness got between us for our marriage to survive.  But all the evil in this world cannot destroy love and truth, and our love is true.  Some say that love equals respect.  Our mutual respect combined with our mutual and primary goal of making this world a better place survives stronger than ever, allowing us to continue working together always even better than before as understanding grows.

During my involuntary unemployment and his involuntary employment, Michael supported me just like in the picture of us in Juan Diego’s cloak from almost 500 years ago that is now hanging in the Basilica of Our Lady of Guadalupe in the north of Mexico City.  I researched, read, studied, and wrote for years.  I read and studied the Word.  I paid professionals to hypnotize me and teach me spirit releasements.  I read their books and their predecessors’ books.  I learned that 90% of societies worldwide have records of spirit attachment phenomena.  I figured out how to share my experience to help others achieve what I have achieved.  Michael remains the best reader I ever met.  He is my first reader, my highest standard.

I finished writing these Instructions for Helping to Improve the Human Condition about four years ago, and Michael and I have used them extensively to better handle personal losses and traumas, deaths of loved ones, even my PTSD.  The journey for both of us has involved extensive self-discovery and personal growth.  We have gotten better and our lives have gotten better.  Therefore, we want to share what we have learned with you.  What else would Mary want but for you to know her Child?  For the idea of God enters all religion and affects conjunction with God, and by conjunction, salvation.

I offer all this information and more for free on my websites.  I am publishing this book to empower more people, especially in the aftermath of the devastation of Hurricane Harvey, which was previewed to us when we were using these Instructions for Helping to Improve the Human Condition months prior to the event, as documented on the websites.  This is the ultimate self-help method best done with a friend as it can involve hypnosis.

Love,

Mary

You can buy the book at amazon.com:  http://a.co/77UxP8a

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